Fig. 3

Differentiated CTX0E16 neurons display morphological features of pyramidal neurons. a-c Generation of small neurites in βIII Tubulin (Tuj1)-positive CTX0E16 cells after 2 or 4 days of differentiation. d-f Expression of green fluorescent protein (GFP) in young CTX0E16 neurons, reveals the development of neuronal morphology; at differentiation day (DD) 15, neurite processes extend from the cell’s soma (d). By DD 20, a single long and thin process can be seen emerging from the cell’s soma with a thicker single process also emerging from the opposite side. Note the pyramidal shape of the cell soma. f By DD 35, the dendritic process displays some level of arborisation; additional smaller processes protruding from the cell soma are also evident. g, h Double immunostaining of DD 35 CTX0E16 neurons for the trans-Golgi marker, GM130 and MAP2. In MAP2-positive neurons, GM130 is clearly seen orientated towards a single, typically the longest, dendrite (red arrows). This indicates the primary dendrite, and the formation of a polarized morphology (g). h High-magnification images reveal that the Golgi-network is present along the primary dendrites (red arrow). Scale bars, 20 μm (a-g) and 5 μm (h). DAPI 4′,6-diamidino-2-phenylindole