Fig. 4

CTX0E16 cells generate neurons that respond to neurotransmitter stimulation. a Expression of a subset of neurotransmitter receptors, synaptic and signalling proteins is increased in differentiation day (DD) 28 CTX0E16 neurons as compared to undifferentiated (DD 0) CTX0E16 neural stem cells (NPCs) indexed by quantitative PCR (q-PCR); n = 4 independent experiments carried out in triplicate; error bars represent SD; **p <0.01; ***p <0.001 (Student’s unpaired t test). b Representative image of neurons loaded with Fura-2 AM used for single cell Ca²⁺ imaging. c-e Representative traces of intracellular Ca²⁺ in response to various neurotransmitter receptor ligands. g Number (%) of total cells generating 5 %, 20 % or 50 % responses following treatment with neurotransmitter ligands; n = 3 independent experiments carried out in triplicate (600 cells in total). h Mean maximal response produced by DD 28 CTX0E16 neurons following application of neurotransmitter ligands; n = 3 independent experiments carried out in triplicate (600 cells in total); error bars represent SD