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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Mesenchymal stem cells derived from human induced pluripotent stem cells retain adequate osteogenicity and chondrogenicity but less adipogenicity

Fig. 6

Gene expression analysis of “stemness” genes in the NHDFs, iPSCs (two lines: mRNA-iPSC and lenti-iPSC), BM-MSCs, mRNA-iPSC-MSC-YL001, lenti-iPSC-MSC-A001 and the three derived lineages (osteogenic, chondrogenic, adipogenic). Expression of eleven stemness genes (POU class 5 homeobox 1 (OCT4/ POU5F1), Kruppel-like factor 4 (KLF4), v-myc avian myelocytomatosis viral oncogene homolog (C-MYC), SRY (sex determining region Y)-box 2 (SOX2), growth differentiation factor 3 (GDF3), teratocarcinoma-derived growth factor 1 (CRIPTO/ TDGF1), undifferentiated embryonic cell transcription factor 1 (UTF1), developmental pluripotency associated 4 (DPPA4), DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), lin-28 homolog A (LIN28A) and spalt-like transcription factor 4 (SALL4)) was analyzed by PCR. a BM-MSCs in growth medium, and after 3 weeks in osteogenic, chondrogenic and adipogenic medium; b NHDF, mRNA-iPSCs, mRNA-iPSC-MSC-YL001 in growth medium, and mRNA-iPSC-MSC-YL001 after 3 weeks in osteogenic, chondrogenic and adipogenic medium; c NHDF, lenti-iPSCs, lenti-iPSC-MSC-A001 in growth medium, and lviPSC-MSC-A001 after 3 weeks in osteogenic, chondrogenic and adipogenic medium. BM bone marrow, iPSC induced pluripotent stem cell, MSC mesenchymal stem cell, NHDF normal human dermal fibroblast

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Stem Cell Research & Therapy

ISSN: 1757-6512