Gene | Primer sequences (5′–3′) | Annealing temperature (°C) | Amplicon (bp) |
---|---|---|---|
OCT4-F | CCTCACTTCACTGCACTGTA | 58 | 163 |
OCT4-R | CAGGTTTTCTTTCCCTAGCT | ||
KLF4-F | GATGAACTGACCAGGCACTA | 60 | 144 |
KLF4-R | GTGGGTCATATCCACTGTCT | ||
C-MYC-F | TGCCTCAAATTGGACTTTGG | 58 | 191 |
C-MYC-R | GATTGAAATTCTGTGTAACTGC | ||
SOX2-F | CCCAGCAGACTTCACATGT | 58 | 150 |
SOX2-R | CCTCCCATTTCCCTCGTTTT | ||
GDF3-F | AAATGTTTGTGTTGCGGTCA | 53 | 178 |
GDF3-R | TCTGGCACAGGTGTCTTCAG | ||
CRIPTO-F | CGGAACTGTGAGCACGATGT | 58 | 65 |
CRIPTO-R | GGGCAGCCAGGTGTCATG | ||
UTF1-F | CCGTCGCTGAACACCGCCCTGCTG | 58 | 179 |
UTF1-R | CGCGCTGCCCAGAATGAAGCCCAC | ||
DPPA4-F | GGAGCCGCCTGCCCTGGAAAATTC | 53 | 407 |
DPPA4-R | TTTTTCCTGATATTCTATTCCCAT | ||
DNMT3B-F | TGCTGCTCACAGGGCCCGATACTTC | 58 | 170 |
DNMT3B-R | TCCTTTCGAGCTCAGTGCACCACAAAAC | ||
LIN28-F | AGTAAGCTGCACATGGAAGG | 58 | 420 |
LIN28-R | ATTGTGGCTCAATTCTGTGC | ||
SALL4-F | GCCGTGAAGACCAATGAGAT | 58 | 243 |
SALL4-R | CTCCTTCCACGCAAGTTCTC | ||
RUNX2-F | CAGTAGATGGACCTCGGGAA | 60 | 188 |
RUNX2-R | CCTAAATCACTGAGGCGGTC | ||
ALP-F | ATCAGGGACATTGACGTGATC | 60 | 137 |
ALP-R | TTCCAGGTGTCAACGAGGTC | ||
COL1A1-F | AGGGCCAAGACGAAGACATC | 60 | 138 |
COL1A1-R | AGATCACGTCATCGCACAAC | ||
OC-F | AGTCCAGCAAAGGTGCAGCC | 60 | 169 |
OC-R | TCAGCCAACTCGTCACAGTC | ||
GAPDH-F | TGGTATCGTGGAAGGACTCATGAC | 53 | 189 |
GAPDH -R | ATGCCAGTGAGCTTCCCGTTCAGC |