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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Isolation, selection and culture methods to enhance clonogenicity of mouse bone marrow derived mesenchymal stromal cell precursors

Fig. 2

Effect of isolation method on surface marker expression and clonogenicity. a Cytograms displaying Sca-1 versus CD45−/Ter119− expression in freshly isolated cBM preparations (left) and FSC versus Sca-1 of cBM CD45−/Ter119− (right). b Histograms showing a comparison of Sca-1 distribution in fBM versus cBM. Values are mean ± SD of at least three experiments. c Evolution of Sca-1, CD44 and CD29 expression on cBM derived CD45− cells in vitro. d CFU-F frequencies among Sca-1 sorted subpopulations of fBM versus cBM. Data are the mean ± SD of at least three independent experiments. *p < 0.05, ***p < 0.001, Student’s t test. cBM compact bone marrow, fBM flushed bone marrow, ns not significant

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Stem Cell Research & Therapy

ISSN: 1757-6512