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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Isolation, selection and culture methods to enhance clonogenicity of mouse bone marrow derived mesenchymal stromal cell precursors

Fig. 3

Effect of oxygen tension on colony counts and size. a CFU-F frequencies in subpopulations from fBM cultured in 21 % oxygen (normoxia) or 5 % oxygen (hypoxia). b CFU-F frequencies in subpopulations from cBM cultured in 21 %, 5 % or 2 % oxygen. Data are the mean ± SD of at least three independent experiments, one-way ANOVA. c Colonies from the indicated populations of sorted cBM. ‘Bulk’ refers to unseparated cells. d Summary of colony size for the different subpopulations in 21 %, 5 % or 2 % oxygen. Data are the means ± standard error of the mean of >100 colonies from at least three independent experiments. *p <0.05, **p <0.01, one-way ANOVA. cBM compact bone marrow, fBM flushed bone marrow, O 2 oxygen

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