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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Stem cells isolated from human dental pulp and amniotic fluid improve skeletal muscle histopathology in mdx/SCID mice

Fig. 1

Cell characterization after cell sorting and evaluation of direct co-culture with C2C12 mouse myoblasts. a Cell characterization after MACS. On the top, fluorescent images of hDPSCs and hAFSCs positively labeled with anti-STRO-1 (red) and anti-c-Kit (green) antibodies, respectively. At the bottom, flow cytofluorimetric analysis of hDPSCs and hAFSCs sorted by MACS; the percentage of positive cells is indicated. b Evaluation of in vitro direct co-culture of hDPSCs and hAFSCs with C2C12 mouse myoblasts. Triple immunofluorescent staining of hAFSCs and hDPSCs co-cultured with C2C12 mouse myoblasts revealed newly formed myotubes which are labeled with DAPI (blue), anti-hNu (green), and anti-MyHC (red) antibodies. C2C12 cells differentiated alone were used as control. Scale bars = 50 μm. DAPI 4′,6-diamidino-2-phenylindole, hAFSC human amniotic fluid stem cell, hDPSC human dental pulp stem cell, hNu human nuclei, MACS magnetic-activated cell sorting, MyHC myosin heavy chain

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Stem Cell Research & Therapy

ISSN: 1757-6512