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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Stem cells isolated from human dental pulp and amniotic fluid improve skeletal muscle histopathology in mdx/SCID mice

Fig. 3

Western blot analysis of muscle-specific markers in differentiated hDPSCs and hAFSCs. Western blot analysis of myogenin and desmin expression in whole cell lysates of differentiated hDPSCs and hAFSCs with (hDPSCs Aza + cm, hAFSCs Aza + cm) and without the addition of C2C12 CM (hDPSCs Aza, hAFSCs Aza). Whole cell lysates were collected from three plates for each experimental group. Actin bands demonstrate that an equal amount of protein was loaded in each lane. Densitometric analysis of Western blot bands is shown at the bottom (analysis of variance followed by Tukey’s test: ***P < 0.001 hDPSCs Aza and hDPSCs Aza + cm versus undiff hDPSCs; ***P < 0.001 hAFSCs Aza and hAFSCs Aza + cm versus undiff hAFSCs; §§§ P < 0.001 hDPSCs Aza + cm versus hDPSCs Aza; §§§ P < 0.001 hAFSCs Aza + cm versus hAFSCs Aza). CM conditioned medium, hAFSC human amniotic fluid stem cell, hDPSC human dental pulp stem cell

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