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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Comparison of adipose tissue- and bone marrow- derived mesenchymal stem cells for alleviating doxorubicin-induced cardiac dysfunction in diabetic rats

Fig. 6

a Bone marrow-derived mesenchymal stem cells (BM-MSCs) and adipose tissue-derived mesenchymal stem cells (AT-MSCs) were labeled with green fluorescent protein (GFP) before injection. Implanted cells were detected in the myocardium 4 weeks after intravenous injection through the tail vein (magnification × 10). b Real-time PCR was performed to quantify transplanted MSCs in the heart. Genomic DNA was isolated from the heart samples, and ALU PCR was performed using TaqMan probes to detect transplanted human MSCs in the rat myocardium. Histograms show percentage of human DNA found in total DNA extracted from rat heart tissues from the BM-MSC and AT-MSC groups. c and d Myocardial sections were stained with Masson’s trichrome, (magnification × 200) to detect fibrosis in the heart in the different groups (control, streptozotocin (STZ), STZ + doxorubicin (STZ + DOX), BM-MSCs and AT-MSCs; d histograms show percent area of collagen deposition. Black arrows indicate collagen fibers. Quantification of the cells in various groups was performed using the Leica Qwin 500 LTD computer-assisted image analysis system (Cambridge, UK). Measurements were done in 10 high power fields (HPF) in all the experimental groups. e and f α-smooth muscle actin (α-SMA) expression was assessed by western blot in different groups. f Histograms show α-SMA levels, values were normalized with glyceraldehyde-3-phosphate dehydrogenase (GAPDH). @ P <0.05 compared to STZ, ▲ P <0.05 compared to STZ and STZ + DOX

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