Table 1 Antibodies used with corresponding working dilutions and origin
CD antigen | Company | Catalogue number | Dilution |
|---|---|---|---|
Brachyury | Abcam (Cambridge, UK) | ab20680 | 1:100 |
CD90 | Abcam (Cambridge, UK) | MRC OX-7 ab225 | 1:100 |
CD105 | Dianova (Hamburg, Germany) | DLN-07243 | 1:100 |
CD73 | Abcam (Cambridge, UK) | 7G2 ab54217 | 1:250 |
CD 34 | Beckman Coulter (Brea, CA, USA) | PN IM1167 | 1:50 |
Snail1 | Santa Cruz (Dallas, TX, USA) | sc-28199 | 1:100 |
MHC class 1 | AbD Serotec (Kidlington, UK) | MCA81 | 1:100 |
Secondary antibodies | Â | Â | Â |
DyLight 488 donkey anti-mouse IgG | Dianova (Hamburg, Germany) | 91518 | 1:400 |
DyLight 549 donkey anti-mouse IgG A | Dianova (Hamburg, Germany) | 88693 | 1:400 |