Fig. 5

hAEC transplantation increased the number of cumulus oocyte complexes (COC) in chemoablated mice. A–C Images of ovarian morphology followed superovulation in different groups 1 month after hAEC transplantation (white arrows). D–F The morphology of COC was observed under microscopy. G Bar graph representing the counting of COC in different groups. H–J H&E staining of ovaries followed superovulation was used to observed follicle development. Enlargements of outlined areas are shown in panels a–f. Mature follicles are indicated by the black arrow (a and e). Primary follicles are indicated by the black arrowhead (b, d and f). Follicular atresia is indicated by the white arrowhead (c). Data are mean ± SEM; *p < 0.05 versus Sham; #p < 0.05 versus Cy. Scale bars = 200 μm (H–J), 100 μm (a, c and e), 50 μm (b, d and f). Cy chemoablated group (n = 4), Cy + hAECs hAEC-treated group (n = 4), Sham sham group (n = 4)