Fig. 2

Human-derived breast cancer was observed in xenotransplantation murine model. a Five weeks after 0.5×10⁶ Fluc-tdT-231 were seeded i.v., NSG mice were injected intraperitoneally with D-Luciferin (150 mg/kg in Dulbecco’s PBS) and in vivo Fluc activity was measured with IVIS Lumina 10 minutes after substrate administration. Exposure time = 5 s. Color scale: minimum = 5.13×10⁷, maximum = 2.46×10⁸. b Representative pictures of tumor-free (left) and tumor-bearing (right) lungs. Eight weeks after MDA-MB-231 breast cancer cells or PBS were seeded i.v., NSG mice were euthanized and lungs were harvested. Frozen sections of lungs of c tumor-free mice and d eGFP-231 tumor-bearing mice sacrificed 5 weeks after cancer seeding were stained with anti-eGFP (green), anti-Ki67 (blue), and TOTO-3 (red). Scale bar: 50 μm. eGFP enhanced green fluorescent protein, i.v. intravenously, NSG nonobese diabetic/severe combined immunodeficiency gamma, PBS phosphate-buffered saline