Fig. 2

Growth kinetics (a) and immunophenotypic features (b–h) of mesenchymal stem cells (MSCs) obtained from BM mononuclear cells (MNCs) using a standard protocol for in vitro MSC expansion compared to the growth kinetics of FACS-sorted and in vitro cultured CD13^high CD105⁺ CD45^– BM cells (identified as CD13^high cells). a Bars and vertical lines correspond to median ± 95 % confidence interval values. b–h Notched boxes extend from the 25th to 75th percentile values; the lines in the middle and vertical lines correspond to median values and both the 10th and 90th percentiles, respectively. Normalized median fluorescence intensity (MFI) values are displayed after subtracting the background autofluorescence levels observed for each individual marker-associated fluorochrome channel (arbitrary units scaled from 0 to 262,000). NS not significant (P > 0.05)