Fig. 1

Validation of the HypoxyGluMet gene expression array in breast cancer cell lines. a Overview of HIF-1-gene targets (red), HIF-1α-dependent glycolytic gene targets (purple), and HIF-1α-independent glycolytic gene targets (blue) assayed by the HypoxyGluMet array. For clarity and consistency, this color scheme is employed in each figure. Gene symbols are shown in parentheses. b Breast cancer cell lines T47D, MCF7, HCC1806, HCC1937, and benign epithelial MCF10A cells were cultured overnight under hypoxic or normoxic conditions. RNA was isolated, cDNA was synthesized, and relative expression of HIF-1 nonglycolytic gene targets (red columns), HIF-1-dependent glycolytic gene targets (purple columns), and HIF-1-independent glycolytic gene targets (blue columns) was quantified via the HypoxyGluMet array. Data shown are the mean of two independent experiments; individual samples were performed in duplicate (Gene Card 2, see Materials and methods) or in triplicate (Gene Card 1). Significance was determined using Student’s paired t test on ΔCt values, with two-tailed distribution. Error bars were calculated as the standard error of the mean (SEM). *p ≤0.05, **p ≤0.01