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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Gene expression signatures of breast cancer stem and progenitor cells do not exhibit features of Warburg metabolism

Fig. 5

Differential response of BCSCs and SCs to hypoxia. a Schematic of experimental design. b Breast cancer (n = 2) and benign tissue (n = 2) specimens were enzymatically digested into single cell suspension. Cells were divided equally and cultured overnight in normoxic or hypoxic conditions. BCSC and SC populations (CD49f+CD24+, CD49f+CD24, CD49fCD24+, CD49fCD24) were immediately isolated by FACS analysis of all cells (adherent and suspension) the following day, and gene expression was determined via the HypoxyGluMet array. Error bars represent of two samples, run in duplicate (Gene Card 1) or in triplicate (Gene Card 2). Significance was determined by Student’s paired t test with two-tailed distribution. **p <0.01. c, d Gene expression of HIF-1α-dependent genes (red), HIF-1α-dependent glycolytic genes (purple), and HIF-1α-independent glycolytic genes (blue) was determined in hypoxic-treated relative to normoxic-treated c benign and d tumor specimens. Error bars (present in all panels but possibly too small to visualize) represent standard deviation of the mean. Significance was determined by Student’s paired t test with two-tailed distribution. *p ≤0.05. BCSC breast cancer stem/progenitor cell, SC benign breast stem/progenitor cell

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