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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: In vivo hepatogenic capacity and therapeutic potential of stem cells from human exfoliated deciduous teeth in liver fibrosis in mice

Fig. 5

SHED-derived HLA-ABC-positive cells purified from primary recipient livers of CCl4-treated mice express hepatocyte-specific genes without host-cell fusion. a Double-immunofluorescent staining patterns for HepPar and human albumin (hALB) in CCl4-injured liver tissues transplanted with SHED. b Flow cytometric analysis of magnetically sorted HLA-ABC-positive (HLA +) cells stained with PE-conjugated anti-human HLA-ABC and APC-conjugated anti-mouse H-K2b antibodies. c Morphology of sorted HLA+ cells. Toluidine blue staining. d Genomic DNA assay. e RT-PCR analysis of hALB and mouse albumin (mALB) mRNAs. f RT-PCR analysis of human hepatocyte-specific genes. ALB albumin, Alu human-specific Alu gene, CCl 4 carbon tetrachloride, CYP1A1 cytochrome P450 1A1, CYP3A7 cytochrome P450 3A7, DAPI 4′,6-diamidino-2-phenylindole, FAH fumarylacetoacetate hydrolase, GAPDH human glyceraldehyde 3-phosphate dehydrogenase, HepG2 human hepatoma cell line, HepPar1 human hepatocyte specific HepParaffin 1 antigen, HLA human leukocyte antigen, HLA HLA-ABC-negative cells, mpf1 mouse-specific Pf1 gene, SHED stem cells from human exfoliated deciduous teeth, TAT tyrosine aminotransferase, TF transferrin, TTR transthyretin, UGT1A1 uridine 5′-diphospho-glucuronosyltransferase 1A1

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