Fig. 1

The expression of miR-17 in CB hematopoietic CD34⁺CD38⁻/CD38⁺ cells. a The expression level of miR-17 in CB CD34⁺CD38⁻/CD38⁺ cells was evaluated by real-time PCR. Each reaction was performed in triplicate. Student’s t-test was used for the statistical analysis between the two indicated populations. b Real-time PCR was performed to evaluate the expression level of miR-17 in CB CD34⁺ cells after transfection with vectors for miR-17 overexpression (17/OE), miR-17 knockdown (17/KD or 17/KD1), or control (CTRL). The data are presented as the ratio of miR-17 levels (relative to U6) in 17/OE, 17/KD or 17/KD1 to that in CTRL. Each reaction was performed in triplicate. c The expression level of miR-17 in 17/OE CD34⁺ cells or 17/KD CD34⁺ cells 5 days after sorting was evaluated by real-time PCR. d The expression level of miR-17 in 17/OE CD34⁺CD38⁻/CD38⁺ cells or 17/KD CD34⁺CD38⁻/CD38⁺ cells 20 days after culture was evaluated by real-time PCR