Fig. 2

The effect of miR-17 modulation on the expansion of CB CD34⁺CD38⁻/CD38⁺ cells. a The expansion of CB CD34⁺ cells upon miR-17 modulation after culturing for 20 days. CB CD34⁺ cells were transfected with vectors for miR-17 overexpression (17/OE), miR-17 knockdown (17/KD), or control (CTRL) and cultured in cytokine-driven serum-free medium. Fold expansion is shown as mean + SD (n = 6). *p < 0.05, between 17/OE or 17/KD, and CTRL group (Student’s t-test). b CB CD34⁺ cells, transfected with ectopic miR-17 vector (17/OE or 17/KD), were further separated through the analysis of CD38 expression. Cells were cultured in cytokine-driven serum-free medium and the cell numbers were monitored. The data are presented as the fold expansion (n = 6, mean + SD). *p < 0.05; **p < 0.01, between 17/OE or 17/KD and CTRL group (Student’s t-test). c Colony forming assays of CB CD34⁺ cells upon miR-17 modulation. After 7 or 14 days of culture, the indicated cell numbers were plated for colony forming assays. The colonies, including BFU-Es, CFU-GMs, and CFU-Mix’s, with greater than 50 cells were counted after 14–16 days. The results are expressed as mean + SD (n = 6). *p < 0.05, between 17/OE or 17/KD, and CTRL group (Student’s t-test). CFU colony forming unit