Fig. 1

Morphology and phenotype of dendritic cells (DCs) generated from the two sources: Phase contrast images of umbilical cord blood DCs (UCB-DCs) (a, b) and peripheral blood PBL DCs (PBL-DCs) (e, f) at lower and higher magnification, respectively. Both sources generated the typical clusters of mature DCs. Wright-Giemsa-stained adherent cell clusters of UCB-DCs (c) and PBL-DCs (g) at lower magnification and morphology of single cells at higher magnification (d, h). FACS histogram overlay profile of a representative experiment from UCB-DCs (i) and PBL-DCs (j). Black line isotype control and colored lines specific CD molecules. Percent expression and mean fluorescence intensity (MFI), respectively, from three samples are shown in (k, l). It is evident that fully mature DCs were generated and there was no significant difference in the expression level of different surface markers, except for CD40, which was higher in UCB-DCs (k). In terms of MFI, CD58 was found significantly higher in UCB-DCs and CD54 in PBL-DCs (l). The data shown are mean ± SD, n = 3 (*p <0.05). PE phycoerythrin, FITC fluorescein isothiocyanate