Fig. 3

Functional characterization of CTLs: (a) Dot plot depicting the gating strategy and expression of activation markers CD69 & CD25 for a representative experiment from UCB/PBL. The dual positive cells from pulsed UCB/PBL-DCs are seen in upper right quadrant of lower panel. (b) Showing significantly higher CD69 & CD25 dual positive cells in UCB-derived CTLs than that of PBL-derived CTLs. (c) The gating strategy for intracellular staining of granzyme A & B in CTLs. Upper right quadrant in lower panel shows dual positive cells. (d) Showing presence of dual positive cells for granzyme A & B in pulsed UCB/PBL derived CTLs. (e) Dot plot and histogram showing the presence of MUC1 STAPPVHNV-specific T cells (lower panel) in total pool of CTLs from both the sources. (f) Showing significantly higher percentage of MUC1 specific TCRs in pulsed PBL/UCB-CTLs set as compared to unpulsed set. MUC1 specific TCRs in pulsed UCB-CTLs set was significantly higher than the PBL counterpart. (g) UCB-CTLs were capable of secreting significantly higher levels of IFN-γ than that of PBL-CTLs (n=5) in the co-culture derived from pulsed UCB/PBL-DCs. (h) Represents the levels of IL-10 and IL-12p70 in the co-culture. The UCB-DCs secreted significantly higher level of IL-12p70 and a significantly lower level of IL-10 than PBL-DCs. (I) IL-12 p70/IL-10 ratio in the co-culture. Ratio was significantly higher in cultures with UCB-DCs as compared to PBL DCs. The data shown are mean ± SD, n = 3/5 (*p < 0.05, **p < 0.01, ***p < 0.001)