Fig. 1
Cell proliferation and alkaline phosphatase (ALP) activity in two-dimensional (2D) cell culture. Cells were plated at the density of 5 × 103/cm2, changed into osteogenic medium and cultured up to 28 days in 2D culture. a Cell enumeration was performed on days 1, 7, 14, 21 and 28. b ALP activity was measured spectrophotometrically in each cell culture well at days 4, 7, 14 and 21. CTRL control, DIFF differentiation, FCS fetal calf serum, ns not significant, PLP platelet lysate and plasma