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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Osteogenic differentiation of mesenchymal stromal cells in two-dimensional and three-dimensional cultures without animal serum

Fig. 2

Quantitative RT-PCR in two-dimensional (2D) cell culture. The cells were cultured in 2D osteogenic medium up to 21 days. Total RNA was isolated and the expression of Runx2 (a), alkaline phosphatase (ALP) (b), collagen I α1 (Col1α1) (c), and osteocalcin (OCN) (d) was analyzed using quantitative RT-PCR. DIFF differentiation, FCS fetal calf serum, PLP platelet lysate and plasma

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Stem Cell Research & Therapy

ISSN: 1757-6512