Fig. 3From: Osteogenic differentiation of mesenchymal stromal cells in two-dimensional and three-dimensional cultures without animal serumDetection of collagenous matrix in 2D cell culture. Cells were cultured in 2D osteogenic conditions up to 21 days in FCS and PLP culture media. At days a 14 and b 21, the cell cultures were fixed and stained with Sirius Red, after which they were dried and photographed. c Thereafter, they were turned into binary images and optical densities were measured. CTRL control, DIFF differentiation, FCS fetal calf serum, PLP platelet lysate and plasmaBack to article page