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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Osteogenic differentiation of mesenchymal stromal cells in two-dimensional and three-dimensional cultures without animal serum

Fig. 4

Detection of mineralized matrix and calcium deposition in two-dimensional (2D) cell culture. Cells were cultured in 2D osteogenic conditions up to 21 days in FCS and PLP culture media. On days a 14 and b 21, the cell cultures were fixed and stained with Alizarin Red, after which they were dried and photographed. c Thereafter, they were turned into binary images and optical densities were measured. d Deposited calcium was dissolved in HCl and quantified on days 7, 14 and 21 of culture using an o-cresolphthalein complexone reagent using a standard curve as reference. CTRL control, DIFF differentiation, FCS fetal calf serum, PLP platelet lysate and plasma

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