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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Urokinase-type plasminogen activator receptor interaction with β1 integrin is required for platelet-derived growth factor-AB-induced human mesenchymal stem/stromal cell migration

Fig. 3

uPA/uPAR mediate PDGF-AB-induced BM-MSC migration. a, b Scratch test assay was performed on BM-MSC isolated from three different donors and seeded in plates coated with type I dermal collagen. Pictures of scratches were taken immediately (T = 0) or 22 hours after culture (T = 22 h) in serum-free control medium or PDGF-AB supplemented medium in the presence or not of anti-uPAR or anti-uPA neutralizing antibodies. a An experiment representative of three experiments. b Quantification of BM-MSC migration. c, d Scratch test assay was performed on BM-MSC isolated from three different donors transfected with either control siRNA (si Neg) or uPAR siRNA (si uPAR) and grown on type I dermal collagen in serum-free control medium or treated with PDGF-AB. c An experiment representative of three experiments. d Migration quantification of transfected BM-MSC. b, d Results expressed as percentages of wound closure at T = 22 h compared with T = 0. Mean of three independent experiments ± SEM are represented (one donor per experiment), each performed in triplicate. *P <0.05. Scale bars: 200 μm. PDGF-AB platelet-derived growth factor AB, si Neg nontargeting small interfering RNA, si uPAR uPAR-targeted small interfering RNA, uPA urokinase-type plasminogen activator, uPAR urokinase-type plasminogen activator receptor

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