Fig. 3

Multiple treatments with cytotoxic drugs influence both osteoblasts and HSPCs in a mouse model. a Osteocalcin mRNA levels were significantly decreased after four cycles of cyclophosphamide treatment, with or without supportive G-CSF therapy (n = 5–8 each group). b Representative scatter plots showing the CD45⁻Ter119⁻OPN⁺ osteoblast population isolated from C57Bl/6 mice. c Number of CD45⁻Ter119⁻OPN⁺ cells from the femurs of C57Bl/6 mice after four cycles of cyclophosphamide treatment, with or without supportive G-CSF therapy (n = 4–6 each group). d Serum osteocalcin concentrations decreased after four cycles of cyclophosphamide treatment, with or without supportive G-CSF. The osteocalcin levels were measured by ELISA (n = 6–8 each group). e H&E staining of sections of decalcified proximal femur from the untreated, CTLs and Gs groups (original magnification, ×40). f The number of HSPCs (colony-forming cells) in the bone marrow of C57Bl/6 mice reduced significantly (n = 6 each group)