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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Human cord blood-derived platelet lysate enhances the therapeutic activity of adipose-derived mesenchymal stromal cells isolated from Crohn’s disease patients in a mouse model of colitis

Fig. 1

adCD-MSCs show typical MSC biological properties. a Representative field of exponential growing culture of adCD-MSCs. b Alizarin red staining of adCD-MSCs cultured for 3 weeks in osteogenic conditions and c Oil red O staining of adCD-MSCs cultured for 3 weeks in adipogenic conditions. Magnification 10×; scale bar, 100 μm. d Comparison of cumulative population doublings (CPD) calculated in adipose tissue-derived MSC cultures obtained from healthy donors (adHD-MSC) or adipose tissue-derived MSC cultures obtained from CD patients (adCD-MSC) at each passage. Results expressed as the mean ± SEM calculated from data obtained from four independent samples. Differences are not significant (p value not significant). e–i Flow cytometric immunophenotype of adCD-MSCs at passage 3. Representative dot plots. The analysis shows a predominant population of cells positive for CD90, CD29, CD73, CD105, CD13, and CD44, and negative for CD34, CD45, CD14, and HLA-DR. FITC, fluorescein isothiocyanate, PE phycoerythrin

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Stem Cell Research & Therapy

ISSN: 1757-6512