Fig. 5
MSC-CM induces TM primary cell survival and contractility. Effect of MSC-CM on human trabecular meshwork (hTM) cell survival and contractility with or without transforming growth factor beta 2 (TGF-β 2 ). hTM cell viability assessed by ATP quantification following 24 hours of increasing BAC concentration exposure with or without MSC-CM a. Immunoblot of phospho-Akt (p-Akt), ± MSC-CM, and phospho-myosin (p-MLC), ± MSC-CM and ± TGF-β2, protein expression using WB in hTM cells was performed b, c. The relative density of protein bands were quantified, and the ratios of phosphorylated protein to actin were calculated (n = 4) b, c. *p <0.05 and ***p <0.001 vs. control culture condition, #p <0.01 vs. TGF-β2 condition. MSC-CM conditioned medium of mesenchymal stem cells