Fig. 2

Pro-inflammatory cytokines induce the upregulation of immune modulatory genes by rMAPC. a Gene expression of immunomodulatory molecules by rMAPC following treatment with respective inflammatory cytokines. Fold differences compared to control (PBS treated, dotted line) are shown. Values represent mean ± SEM from five independent experiments. b Measurement of nitrite in the culture supernatant of rMAPC. Concentration of nitrite is shown (μM). Mean values (±SEM) from four independent experiments. c Expression of Ido-1 following 12 hours treatment with respective cytokines. YWHAZ and HMBS were used as loading controls. Representative experiment out of n = 5 performed. Non-parametrical Kruskal Wallis multiple group comparison test was used followed by Dunns test for differences between groups. Significant differences with the control condition are indicated with asterisks: *P ≤ 0.05, ** P ≤ 0.01, ***P ≤ 0.001. COX cyclooxygenase, HMBS hydroxymethylbilane synthase, IFN interferon, ido-1 indoleamine 2,3-dioxygenase 1, IL interleukin, iNOS inducible nitric oxide synthase, PBS phosphate-buffered saline, PD-L1 programmed death-ligand 1, TNF tumor necrosis factor, TSG TNF stimulated gene, YWHAZ 14-3-3 protein zeta/delta