Fig. 5

Inflammatory treatment enhances the chemoattractive activity of rMAPC. a Gene expression of chemokines by rMAPC following inflammatory treatment. Fold differences compared to control (PBS treated, dotted line) are shown. Significant differences with the control condition are indicated with asterisks. Values represent mean ± SEM from five independent experiments. b Migration of MBP-specific T cells towards supernatants derived from licensed rMAPC (with defined cytokines combinations). Chemotactic index is shown, which is calculated by dividing the number of migrated T cells per condition by the number of migrated T cells of the negative control (non-conditioned medium). Results are from three independent experiments, with duplicates per experiment. c Detection of chemokine receptor mRNA expression in MBP-specific T cells. The genes selected have as ligands the chemokines upregulated in panel a. The picture is a representative experiment out of a total of three independent experiments. Non-parametrical Kruskal Wallis multiple groups comparison test was used followed by Dunns test for differences between groups. Significant differences with the control condition are indicated with asterisks: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. IFN interferon, IL interleukin, rMAPC s/n supernatant derived by naïve (non-treated) rat multipotent adult progenitor cells, TNF tumor necrosis factor