Fig. 6

rMAPC migration is altered following licensing. a Detection of chemokine receptor mRNA in rMAPC. Amplified cDNAs are visualized in a 1.5 % agarose gel. YWHAZ and HMBS were used as loading controls. One representative experiment out of five is shown. b rMAPC migration towards important MS pathogenesis chemokines. Three different concentrations of chemokines were used in the lower compartment (100, 250 and 500 ng/ml). The percentage of area covered by the migrated cells is shown. Values represent mean ± SEM from four independent experiments, with duplicates per experiment. Asterisks show statistical significance between the different concentrations of each chemokine and the negative control. No statistically significant differences were found between the different concentrations for each chemokine. Positive control values showed statistically significant differences with all the other conditions. Data were analyzed with one way analysis of variance comparison test, followed by Dunnett’s test for differences between the groups. c Gene expression of chemokine receptors by rMAPC following inflammatory treatment. Fold differences compared to control (PBS treated, dotted line) are shown. Significant differences with the control condition are indicated with asterisks. Values represent mean ± SEM from five independent experiments. d rMAPC migration following 12 hours of cytokine pre-treatment. Three different concentrations of chemokines were used in the lower compartment (100, 250 and 500 ng/ml). Only the statistically significant changes are shown here. The percentage of area covered by the migrated cells is shown. Values represent mean ± SEM from four independent experiments, with duplicates per experiment. Asterisks show the statistical significant difference between the pre-treated and not treated (naïve) migrated fraction towards specific concentrations of each chemokine. All values differ significantly with n.c. Mean of positive control is statistically significant to all the other conditions. Data were analyzed with one way analysis of variance comparison test, followed by Dunnett’s test for differences between the groups. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. IFN interferon, IL interleukin, n.c. negative control, p.c. positive control, rMAPC rat multipotent adult progenitor cells, TNF tumor necrosis factor