Fig. 4
Exogenous H2O2 suppressed the expression of Olig2, SOX2, and CD133 in GSCs. a Exogenous H2O2 caused an increase in mitochondrial O2 − in GSC11 cells. Cells were treated with 200 μM or 1 mM of H2O2 for 1 h, and mitochondrial O2 − was measured by flow cytometric analysis. b Exogenous H2O2 caused an increase in mitochondrial O2 − in GSC23 cells. Cells were treated with 500 μM or 1 mM of H2O2 for 1 h, and mitochondrial O2 − was then measured by flow cytometric analysis. c GSC11 cells were treated with 200 μM or 1 mM H2O2 for 6 h with addition of H2O2 at 1-h intervals. Expression of SOX2, Olig2, and CD133 mRNA in GSC11 cells was measured by quantitative reverse transcription-polymerase chain reaction. *P < 0.05. d Effect of exogenous H2O2 on the expression of SOX2, Olig2, and CD133 in GSC23 cells under the same conditions as in (c). *P < 0.05. Cont control, GSC glioma stem cell, H 2 O 2 hydrogen peroxide, O 2 − superoxide