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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Pre-differentiation of human neural stem cells into GABAergic neurons prior to transplant results in greater repopulation of the damaged brain and accelerates functional recovery after transient ischemic stroke

Fig. 4

Undifferentiated hNSCs and predifferentiated cell phenotypes in vivo. Confocal immunofluorescent photomicrographs of undifferentiated hNSCs at 28 days post transplant within cortical border regions with orthogonal reconstructions. Undifferentiated hNSCs maintained expression of HuNu (red) and colabeled mainly with GFAP (a), Nestin (b), and Ki67 (c), with little expression of GABA (d) and Tuj1 (e) (green). Immunofluorescent confocal images of predifferentiated cells 28 days post transplant within the stroke-damaged cortex maintained expression of HuNu (red) colabeled with Tuj1 (f), GABA (g), Nestin (h), and Ki67 (i) (green), with lack of colocalization with GFAP (green) (j). Orthogonal reconstructions from confocal z-series are presented as viewed in x–z (top) and y–z (right) planes. HuNu immunoreactivity of undifferentiated hNSCs was observed completely surrounded by GFAP and Nestin, while HuNu immunoreactivity of predifferentiated cells was observed completely surrounded by Tuj1 and GABA. Scale bar: (a–e) 50 μm, orthogonal images 5 μm; (f–j) 20 μm, orthogonal images 5 μm. GABA gamma-aminobutyric acid, GFAP glial fibrillary acidic protein, HuNu human specific nuclear antigen, Tuj1 β-III tubulin

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