Fig. 5

Phenotypic profile of transplanted undifferentiated hNSCs versus predifferentiated cells. Coronal sections immunostained with NeuN (green) highlight undamaged brain regions with lack of staining within stroke affected areas (white dotted line) at cortical graft sites +1.4 mm and –2.16 mm relative to the bregma (a, c) and striatal graft sites +1.4 mm and +0.2 mm relative to the bregma (e, g); white boxes depict graft location. Representative images of graft sites stained with HuNu (red) and NeuN (green) (b, d, f, h) from regions highlighted by white boxes (a, c, e, g). Numbers of HuNu-positive cells coexpressing Tuj1, GABA, Nestin, GFAP, and Ki67 from undifferentiated hNSC-treated (n = 7) and predifferentiated cell-treated (n = 7) animals from cortical graft sites; +1.4 mm (i) and –2.16 mm (j) relative to the bregma; and striatal graft sites +1.4 mm (k) and +0.2 mm (l) relative to the bregma. Numbers of cells are presented as a percentage of the total number of HuNu-positive cells counted. Data are mean ± SEM. ***P <0.001, ****P <0.0001 compared with undifferentiated hNSC counts (two-way ANOVA with Bonferroni post test). Scale bar: (a, c, e, g) 2000 μm, (b, d, f, h) 200 μm. GABA gamma-aminobutyric acid, GFAP glial fibrillary acidic protein, HuNu human specific nuclear antigen, NeuN neuron specific nuclear antigen, Tuj1 β-III tubulin