Fig. 6From: Pre-differentiation of human neural stem cells into GABAergic neurons prior to transplant results in greater repopulation of the damaged brain and accelerates functional recovery after transient ischemic strokeFurther maturation of predifferentiated cells 28 days post transplant. Confocal photomicrographs of predifferentiated cells 28 days post transplant within the stroke-damaged brain expressed HuNu (red) double labeled with either GAD65/67 (green) (a), calbindin-D28k (CB; green) (b), or calretinin (CR, green) (c). Predifferentiated cells expressing HuNu (blue) triple-labeled with parvalbumin (PV, green) and GABA (red) (d), or triple-labeled with Tuj1 (red) and presynaptic vesicle protein synaptophysin (SYN, green) within the cortical core (e) and border (f) regions. Some predifferentiated cells expressing HuNu (red) grafted to border regions appeared to extend long neurites expressing Tuj1 (green) (g). Orthogonal reconstructions from confocal z-series are presented as viewed in x–z (top) and y–z (right) planes. Scale bar: (a, b) 10 μm, orthogonal image 5 μm; (c, d) 40 μm, orthogonal image 5 μm; (e, f) 10 μm, orthogonal image 5 μm; (g) 20 μm, orthogonal image 5 μm. GABA gamma-aminobutyric acid, GAD glutamate decarboxylase 65&67, HuNu human specific nuclear antigen, Tuj1 β-III tubulinBack to article page