Rapid and high-efficiency generation of mature functional hepatocyte-like cells from adipose-derived stem cells by a three-step protocol

Table 1 Hepatic lineage differentiation condition

	Induction period	Medium contents
Endodermal induction	Days 0–1	RPMI-1640, IDE1 (100 nM), CHIR99021 (3 μM)
Hepatogenic induction	Days 2–4	RPMI-1640, IDE1 (100 nM), FGF4 (20 ng/ml), HGF (150 ng/ml)
Maturation step	Days 5–9	Williams’ E, HGF (50 ng/ml), EGF (20 ng/ml), OsM (30 ng/ml), Dex (2 × 10⁻⁵ M), ITS (1×)

DEX dexamethasone, EGF epidermal growth factor, FGF4 fibroblast growth factor 4, HGF hepatocyte growth factor, ITS insulin–transferrin–selenium, OsM Oncostatin M

ISSN: 1757-6512