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Table 1 Hepatic lineage differentiation condition

From: Rapid and high-efficiency generation of mature functional hepatocyte-like cells from adipose-derived stem cells by a three-step protocol

  Induction period Medium contents
Endodermal induction Days 0–1 RPMI-1640, IDE1 (100 nM), CHIR99021 (3 μM)
Hepatogenic induction Days 2–4 RPMI-1640, IDE1 (100 nM), FGF4 (20 ng/ml), HGF (150 ng/ml)
Maturation step Days 5–9 Williams’ E, HGF (50 ng/ml), EGF (20 ng/ml), OsM (30 ng/ml), Dex (2 × 10−5 M), ITS (1×)
  1. DEX dexamethasone, EGF epidermal growth factor, FGF4 fibroblast growth factor 4, HGF hepatocyte growth factor, ITS insulin–transferrin–selenium, OsM Oncostatin M