Fig. 4

Bone marrow mononuclear cell invasive function can be measured by ECIS. a ECIS Transwell filter resistance increases over 2 hours as a result of porcine BM-MNC invasion of Matrigel in response to SDF-1. Without the addition of SDF-1 to the lower chamber, the porcine BM-MNCs (added to the upper chamber) did not invade the Matrigel, resulting in no increase in the resistance (lower tracing). Arrow: time point of SDF-1 addition to test lower chambers. Each tracing represents the mean ± SEM of results from four separate experiments. b The absolute change in ECIS Transwell filter resistance, relative to the resistance measured at the zero time point, increases over 2 hours due to porcine BM-MNC Matrigel invasion in response to SDF-1. Each bar represents the mean ± SEM of separate BM-MNC isolations from four different animals (n = 4). *p <0.05. SDF-1 stromal cell derived factor-1