Fig. 5

ECIS measurement of decreased PB-MNC invasive function resulting from radiation exposure. PB-MNCs were irradiated (2.15 Gy) or not irradiated, cultured for 24 hours, and then added to the upper chambers of ECIS Transwells, with 100 ng/ml SDF-1 and 100 ng/ml MIP-1 chemokines added to the appropriate bottom chambers at time zero (arrow). a Resistance measurement tracings over time demonstrating the loss of invasive function by irradiated PB-MNCs. Mean ± SEM. b The absolute change in ECIS Transwell filter resistance, relative to the resistance measured at the zero time point, is decreased by irradiation of PB-MNCs. Each bar represents the mean ± SEM of separate PB-MNC isolations from two different animals (n = 2). *p <0.05. ECIS electric cell-substrate impedance sensing