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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Roles of microRNA-34a targeting SIRT1 in mesenchymal stem cells

Fig. 3

miR-34a induces apoptosis by modifying SIRT1 and FOXO3a expression. a, b Apoptosis was analyzed by measuring Annexin V+/PI cells using flow cytometry in cultures of siRNA-SIRT1, siRNA-NT, or siRNA-SIRT1 cotransfected with miR-34a inhibitor-treated MSCs, under normal and H/SD conditions (MSCs were transfected for 72 hours and exposure to H/SD and maintained as such for 6 hours). *P <0.05 vs. normal siRNA-NT, P < 0.05 vs. H/SD siRNA-NT. c, d MSCs were transfected with miR-34a mimic, NC mimic, siRNA-SIRT1, or siRNA-NT for 72 hours, respectively, and then CASP3 and PARP1 activity was measured using western blot. *P <0.05 vs. NC mimic, P <0.05 vs. siRNA-NT. e, f Western blot analysis of SIRT1, FOXO3a, Bim, CASP3, and PARP1 protein expression in cultures of siRNA-NT, siRNA-SIRT1, miR-34a inhibitor, or siRNA-SIRT1 cotransfected with miR-34a inhibitor-treated MSCs, under normal and H/SD conditions (MSCs were transfected for 72 hours and exposure to H/SD and maintained as such for 6 hours). β-actin was used as the internal control. Each column represents mean ± SD from three independent experiments. *P <0.05 vs. normal scramble, P <0.05 vs. H/SD scramble. CASP3 caspase 3, FOXO3a forkhead box O transcription factor 3a, H/SD hypoxia and serum deprivation, miRNA microRNA, NC negative control, PARP1 polyADP-ribose polymerase 1, PI propidium iodide, SIRT1 silent information regulator 1, siRNA small interfering RNA, siRNA-NT scrambled siRNA

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