Fig. 5
From: Roles of microRNA-34a targeting SIRT1 in mesenchymal stem cells
Overexpression of miR-34a induces senescence in MSCs. Cells were left untreated or pretreated with miR-34a mimic, siRNA-SIRT1, ROS scavenger NAC (10 mM), and miR-34a inhibitor separately or in combination for 72 hours, and then cellular senescence was analyzed by SA-β-gal staining (a, b). Cellular ROS production was assessed by measuring the fluorescent intensity of DCFH-DA determined using flow cytometry (c, d). Cellular DNA damage and senescence-related proteins including γ-H2A.X, p53, p21, and p16 were determined with western blot (e, f). Each column represents mean ± SD from three independent experiments. *P <0.05 vs. scramble, △P <0.05 vs. miR-34a mimic, ▲P <0.05 vs. siRNA-SIRT1.cp. DCFH 2′,7′-dichlorodihydrofluorescein, MFI mean fluorescence intensity, miRNA microRNA, NAC N-acetylcysteine, SA-β-gal senescence-associated galactosidase, SIRT1 silent information regulator 1, siRNA small interfering RNA