Table 1 Primers for quantitative RT-PCR and oligonucleotides
From: Roles of microRNA-34a targeting SIRT1 in mesenchymal stem cells
Name | Â | Sequence |
|---|---|---|
Quantitative RT-PCR | Â | Â |
 miR-34a | Forward | 5′-AAGGCCACGGATAGGTCCATA-3′ |
|  | Reverse | 5′-CGCTTTGGTGGTTCTGAAAGG-3′ |
 SIRT1 | Forward | 5′-AAGGCCACGGATAGGTCCATA-3′ |
|  | Reverse | 5′-CGCTTTGGTGGTTCTGAAAGG-3′ |
 FOXO3a | Forward | 5′-TGCCGATGGGTTGGATTT-3′ |
|  | Reverse | 5′-CCAGTGAAGTTCCCCACGTT-3′ |
 U6 | Forward | 5′-AAGGCCACGGATAGGTCCATA-3′ |
|  | Reverse | 5′-CGCTTTGGTGGTTCTGAAAGG-3′ |
 β-actin | Forward | 5′-CCCAGCACAATGAAGATCAAGATCAT-3′ |
|  | Reverse | 5′-ATCTGCTGGAAGGTGTACAGCGA-3′ |
Oligonucleotide | Â | Â |
 miR-34a mimic |  | UGGCAGUGUCUUAGCUGGUUGUU CAACCAGCUAAGACACUGCCAUU |
 NC mimic |  | UUCUCCGAACGUGUCACGUTT ACGUGACACGUUCGGAGAATT |
 miR-34a inhibitor |  | UGGCAGUGUCUUAGCUGGUUGUU |
 NC inhibitor |  | CAGUACUUUUGUGUAGUACAA |
 siRNA-SIRT1 |  | GCACCGAUCCUCGAACAAUTT AUUGUUCGAGGAUCGGUGCTT |
 siRNA-NT |  | UUCUCCGAACGUGUCACGUTT ACGUGACACGUUCGGAGAATT |