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Table 1 Primers for quantitative RT-PCR and oligonucleotides
From: Roles of microRNA-34a targeting SIRT1 in mesenchymal stem cells
| Name | Sequence | |
|---|---|---|
| Quantitative RT-PCR | ||
| miR-34a | Forward | 5′-AAGGCCACGGATAGGTCCATA-3′ |
| Reverse | 5′-CGCTTTGGTGGTTCTGAAAGG-3′ | |
| SIRT1 | Forward | 5′-AAGGCCACGGATAGGTCCATA-3′ |
| Reverse | 5′-CGCTTTGGTGGTTCTGAAAGG-3′ | |
| FOXO3a | Forward | 5′-TGCCGATGGGTTGGATTT-3′ |
| Reverse | 5′-CCAGTGAAGTTCCCCACGTT-3′ | |
| U6 | Forward | 5′-AAGGCCACGGATAGGTCCATA-3′ |
| Reverse | 5′-CGCTTTGGTGGTTCTGAAAGG-3′ | |
| β-actin | Forward | 5′-CCCAGCACAATGAAGATCAAGATCAT-3′ |
| Reverse | 5′-ATCTGCTGGAAGGTGTACAGCGA-3′ | |
| Oligonucleotide | ||
| miR-34a mimic | UGGCAGUGUCUUAGCUGGUUGUU CAACCAGCUAAGACACUGCCAUU | |
| NC mimic | UUCUCCGAACGUGUCACGUTT ACGUGACACGUUCGGAGAATT | |
| miR-34a inhibitor | UGGCAGUGUCUUAGCUGGUUGUU | |
| NC inhibitor | CAGUACUUUUGUGUAGUACAA | |
| siRNA-SIRT1 | GCACCGAUCCUCGAACAAUTT AUUGUUCGAGGAUCGGUGCTT | |
| siRNA-NT | UUCUCCGAACGUGUCACGUTT ACGUGACACGUUCGGAGAATT |
