From: Roles of microRNA-34a targeting SIRT1 in mesenchymal stem cells
Name | Sequence | |
---|---|---|
Quantitative RT-PCR | ||
miR-34a | Forward | 5′-AAGGCCACGGATAGGTCCATA-3′ |
Reverse | 5′-CGCTTTGGTGGTTCTGAAAGG-3′ | |
SIRT1 | Forward | 5′-AAGGCCACGGATAGGTCCATA-3′ |
Reverse | 5′-CGCTTTGGTGGTTCTGAAAGG-3′ | |
FOXO3a | Forward | 5′-TGCCGATGGGTTGGATTT-3′ |
Reverse | 5′-CCAGTGAAGTTCCCCACGTT-3′ | |
U6 | Forward | 5′-AAGGCCACGGATAGGTCCATA-3′ |
Reverse | 5′-CGCTTTGGTGGTTCTGAAAGG-3′ | |
β-actin | Forward | 5′-CCCAGCACAATGAAGATCAAGATCAT-3′ |
Reverse | 5′-ATCTGCTGGAAGGTGTACAGCGA-3′ | |
Oligonucleotide | ||
miR-34a mimic | UGGCAGUGUCUUAGCUGGUUGUU CAACCAGCUAAGACACUGCCAUU | |
NC mimic | UUCUCCGAACGUGUCACGUTT ACGUGACACGUUCGGAGAATT | |
miR-34a inhibitor | UGGCAGUGUCUUAGCUGGUUGUU | |
NC inhibitor | CAGUACUUUUGUGUAGUACAA | |
siRNA-SIRT1 | GCACCGAUCCUCGAACAAUTT AUUGUUCGAGGAUCGGUGCTT | |
siRNA-NT | UUCUCCGAACGUGUCACGUTT ACGUGACACGUUCGGAGAATT |