Fig. 2

Transcriptional upregulation of the migratory-related factors by hBMSC with high bone formation capacity. a The transwell migration ability of three high bone-forming (HBF1, HBF2, HBF3) clones versus three low bone-forming (LBF1, LBF2, LBF3) clones toward PDGF-BB (100 ng/ml). Migrated cells presented as percentage of control condition (without chemoattractant). Data presented as mean ± SEM of at least three independent experiments per each clone (***p ≤0.005 as compared with nontreated control; ###p ≤0.005 between the mean value of three HBF clones versus the mean value of three LBF clones). b Heat map of molecular signature of hBMSC-TERT^+Bone, hBMSC-TERT^–Bone, HBF clones (HBF1, HBF2, HBF3), and LBF clones (LBF1, LBF2, LBF3). Hierarchical clustering analysis based on Pearson’s correlation (group gene profile, overall expression data) between different groups shows the clustering of HBF clones with hBMSC-TERT^+Bone and LBF clones with hBMSC-TERT^–Bone. c Annotation analysis of differentially upregulated genes (p <0.01, twofold cutoff) by HBF clones versus LBF clones according to gene molecular function. d Real-time RT-PCR analysis of some upregulated genes (from microarray analysis by HBF clones versus LBF cones). The expression of each target gene was normalized to RG and presented as relative expression to RG. Data presented as mean (three different clones of both HBF and LBF clones) ± SEM of at least three independent experiments (*p ≤0.05, **p ≤0.01, between LBF versus HBF clones). PDGF platelet-derived growth factor, RG reference genes