Table 1 Genes differentially upregulated in HBF clones + hBMSC-TERT^+Bone versus LBF clones + hBMSC-TERT^–Bone

Migration

ILMN_1798360

CXCR7

Chemokine (C-X-C motif) receptor 7

1.57 × 10^–5

Receptor for CXCL12/SDF1

ILMN_1689111

CXCL12

Chemokine (C-X-C motif) ligand 12

6.57 × 10^–5

Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis

ILMN_2373791

ENPP2

Ectonucleotide pyrophosphatase/phosphodiesterase 2

1.58 × 10^–13

Leukocyte-endothelial cell adhesion

ILMN_1722713

FBLN1

Fibulin 1

8.1 × 10^–17

Plays a role in cell adhesion and migration

ILMN_2082585

SNAI2

Snail homolog 2

3.28 × 10^–25

Involved in the generation and migration of neural crest cells

ILMN_1811313

SLIT3

Slit homolog 3

1.14 × 10^–15

Acts as molecular guidance cue in cellular migration

ILMN_1802646

EPHB6

EPH receptor B6

0.00022

Modulates cell adhesion and migration

ILMN_2086470

PDGFRA

Platelet-derived growth factor receptor, alpha polypeptide

0.009945

Receptor that binds both PDGFA and PDGFB

ILMN_2339266

LAMA2

Laminin, alpha 2

5.47 × 10^–6

Mediates the attachment, migration, and organization of cells into tissues during embryonic development

ILMN_2307903

VCAM1

Vascular cell adhesion molecule 1

7.75 × 10^–5

Plays a role in leukocyte endothelial cell adhesion

ILMN_1757338

PLSCR4

Phospholipid scramblase 4

0.008172

Mediates bidirectional transbilayer migration of phospholipids, plays a central role in the initiation of fibrin clot formation and activation of mast cells and in the recognition of apoptotic and injured cells

ILMN_1761540

SEMA3F

Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3 F

8.68 × 10^–8

Plays a role in cell motility and cell adhesion

ILMN_1659306

SVIL

Supervillin

0.001237

May modulate myosin II regulation through MLCK during cell spreading, an initial step in cell migration

ILMN_1667893

TNS3

Tensin 3

5.1 × 10^–6

Involved in cell migration and bone development

ILMN_1815057

PDGFRB

Platelet-derived growth factor receptor

5.33 × 10^–10

Receptor that binds both PDGF-AA and PDGF-BB

Adhesion

ILMN_2408683

PPAP2B

Phosphatidic acid phosphatase type 2B

4.45 × 10^–11

May be involved in cell adhesion and in cell–cell interactions

ILMN_1684554

COL16A1

Collagen, type XVI, alpha 1

4.96 × 10^–12

Involved in mediating cell attachment and induction of integrin-mediated cellular reactions, such as cell spreading and alterations in cell morphology

ILMN_1801246

IFITM1

Interferon induced transmembrane protein 1 (9–27)

1.36 × 10^–14

Component of a complex involved in adhesion signals

ILMN_2396444

CD14

CD14 molecule

0.000265

Upregulates cell surface molecules, including adhesion molecules

ILMN_2307903

VCAM1

Vascular cell adhesion molecule 1

7.75 × 10^–5

Plays a role in leukocyte endothelial cell adhesion

ILMN_2229877

PCDH18

Protocadherin 18

6.56 × 10^–8

Potential calcium-dependent cell-adhesion protein

ILMN_1761540

SEMA3F

Sema domain, immunoglobulin domain (Ig), short basic domain, secreted

8.68 × 10^–8

May play a role in cell motility and cell adhesion

ILMN_1812461

WISP2

WNT1 inducible signaling pathway protein 2

0.001181

Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors

ILMN_1769575

JAM3

Junctional adhesion molecule 3

4.39 × 10^–5

May participate in cell–cell adhesion

ILMN_2223941

FBLN5

Fibulin 5

0.000119

Promotes adhesion of endothelial cells through interaction of integrins

ILMN_2115125

CTGF

Connective tissue growth factor

3 × 10^–9

Mediates heparin-dependent and divalent cation-dependent cell adhesion

ILMN_1707070

PCOLCE

Procollagen C-endopeptidase enhancer

1.28 × 10^–5

Procollagen C-endopeptidase enhancer

Bone formation

ILMN_1696391

LEPR

Leptin receptor

0.000244

Leptin receptor

ILMN_1690945

CPZ

Carboxypeptidase Z

2.46 × 10^–11

Modulates the Wnt signaling pathway

ILMN_1800317

WNT5A

Wingless-type MMTV integration site family, member 5A

5.65 × 10^–6

Can activate or inhibit canonical Wnt signaling

ILMN_1709734

BMP4

Bone morphogenetic protein 4

0.003465

Induces cartilage and bone formation

ILMN_1758895

CTSK

Cathepsin K (CTSK), mRNA.

0.000136

Closely involved in osteoclastic bone resorption

ILMN_1684755

KAZALD1

Kazal-type serine peptidase inhibitor domain 1

0.001055

Involved in the proliferation of osteoblasts during bone formation and bone regeneration

ILMN_1724480

AXIN2

Axin 2

5.5 × 10^–7

Downregulates beta-catenin

ILMN_1770161

BST1

Bone marrow stromal cell antigen 1

0.002254

Involved in osteoclastic bone resorption

ILMN_1729368

FZD8

Frizzled homolog 8

0.005335

Receptor for Wnt proteins

Cytoskeletal

ILMN_1812031

PALM

Paralemmin

0.004851

Control of cell shape

ILMN_1780334

KCNJ2

Potassium inwardly-rectifying channel, subfamily J, member 2

1.23 × 10^–21

Participates in establishing action potential waveform and excitability of neuronal and muscle tissues

ILMN_1654319

HAPLN3

Hyaluronan and proteoglycan link protein 3

3.95 × 10^–27

May function in hyaluronic acid binding

ILMN_1741695

COL12A1

Collagen, type XII, alpha 1

0.008916

ILMN_1670899

FBN2

Fibrillin 2

0.000762

Contains microfibrils that regulate the early process of elastic fiber assembly

ILMN_1674620

SGCE

Sarcoglycan, epsilon

7.35 × 10^–6

A subcomplex of the dystrophin–glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix

ILMN_1780334

KCNJ2

Potassium inwardly-rectifying channel, subfamily J, member 2

1.23 × 10^–21

Participates in establishing action potential waveform and excitability of neuronal and muscle tissues