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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Protease inhibitors enhance extracellular collagen fibril deposition in human mesenchymal stem cells

Fig. 2

Extracellular collagen fibril formation by hMSCs in different treatment groups at different time points. a-d 4 h. e-h 24 h. i-l 48 h. m-p 72 h. q-t Day 11. a, e, i, m, q Control (normal medium). b, f, j, n, r Inhibition of intracellular matrix degradation by intracellular cysteine proteinase inhibitor E64D. c, g, k, o, s Inhibition of extracellular matrix degradation by wide range collagenase inhibitor GM6001. d, h, l, p, t Inhibition of both intracellular and extracellular matrix degradation. E64D (20 μM); GM6001 (25 μM); green: Alexa Fluor 488-labeled collagen type I at 10 μg/ml; orange: Lysosomes. u Quantitative analysis of percentage of collagen content in extracellular fibril fraction under different treatments and at different time points (n = 2 with duplicates). hMSC human mesenchymal stem cell

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