Fig. 2

Treatment with MenSCs alone or in combination with antibiotics improves survival and protects against multiorgan dysfunction in CLP-induced sepsis. a Survival curves in mice with polymicrobial sepsis and different treatments. C57BL6/J mice subjected to CLP-induced sepsis were randomized into five groups: sham (n = 12), saline (n = 16), antibiotics (AB) (n = 22), MenSCs (n = 21), and MenSCs + AB (n = 22). Three hours post-surgery, mice were injected with saline (vehicle), AB and/or MenSCs. Survival percentages of untreated and treated mice after CLP are presented as a Kaplan-Meier survival curve. b Serum concentration of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and glucose after CLP-induced sepsis. Serum was isolated 24 hours post-surgery and treatment administration (sham, n = 3; saline, n = 5; AB, n = 4; MenSCs, n = 4; MenSCs + AB, n = 4), and the concentrations of biomarkers of liver function and total glucose were determined. Dot plots represent individual values, horizontal bars represent mean values, and vertical bars represent standard error values. c Lung histology after CLP-induced sepsis. At 40 hours post-surgery and treatment infusion, lungs were collected, fixed, embedded in paraffin, and stained with hematoxylin-eosin. Pictures are representative images of lungs from the different experimental groups. AB antibiotics, CLP cecal ligation puncture, MenSCs menstrual derived mesenchymal stem cells