Fig. 8
From: Enhanced osteogenic potential of mesenchymal stem cells from cortical bone: a comparative analysis
Osteogenic potential assessed on AD-, BM-, and CBF-MSCs after 4 weeks in induction media. Cells were cultured in normoxic (a) and hypoxic (b) conditions. von Kossa stainings showing mineral deposits produced by the three cell types. Cell grown in standard media for the same period are also showed (second and fourth rows). Magnification: 10×. Scale bar: 40 μm. c Quantification of mineral deposition following osteogenic induction in normoxia and hypoxia represented as a percentage of total well area. Values are mean ± standard deviation (n = 3). Asterisks depict highly significant differences (P < 0.0005) between CBF-MSCs and AD-MSCs and BM-MSCs. AD-MSC adipose tissue mesenchymal stem cell, BM-MSC bone marrow mesenchymal stem cell, CBF-MSC cortical bone fragment mesenchymal stem cells