Skip to main content
Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Differential ability of MSCs isolated from placenta and cord as feeders for supporting ex vivo expansion of umbilical cord blood derived CD34+ cells

Fig. 2

P-MSCs supported robust expansion of CD34+ cells without deterring their quiescence state. a Co-culturing of CD34+ cells with P-MSCs harbored a significantly higher TNC yield, CD34+CD38− and CD133+ cells than C-MSCs as feeders. b Upper panel- A representative FACS dot plot showing higher CD34+CD38− cells with P-MSCs as feeders. Lower panel- A representative FACS histogram depicting total CD34+CD133+ cells in the co-culture C- and P MSCs as feeders. c Cell cycle analysis demonstrated an increase of CD34+ cells at G0/G1 phase with P-MSCs as feeders and higher numbers of cycling CD34+ cells in cultures comprised of C-MSCs as feeders. d FACS dot plot profile of Hoechst and PyroninY based cell cycle analysis showing P-MSCs as feeders had a higher percentage of CD34+ cells in G0 (quiescence) stage in the co-cultures with P-MSCs than C-MSCs. Data are represented as mean ± standard deviation from three independent sets of experiments. *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001 P-MSCs placenta-derived mesenchymal stem cells, C-MSCs cord-derived mesenchymal stem cells, FACS fluorescence-activated cell sorting

Back to article page