Fig. 1

Surface markers and multilineage differentiation capacity of rapamycin-treated and untreated Ad-hMSCs. a Expression of markers in Ad-hMSCs was analyzed by flow cytometry. One representative analysis is reported. From left to right: CD90, CD13, CD44, CD29, CD105 (upper row); CD11b, CD19, CD31, CD34, CD45, HLA-DR (lower row). Red histograms, cells stained with isotype control; blue histogram, cells isolated with specific antibodies. b MSCs were plated on each differentiation medium. After 1 week (adipogenic differentiation) or 3 weeks (osteoblastogenic and chondrogenic differentiation), cells were fixed and stained with FABP-4 (top), osteocalcin (middle), or aggrecan (bottom) and visualized by fluorescence microscopy. Representative images of MSC-induced differentiation into adipogenic, osteogenic, and chondrogenic pathways.. Ad-hMSCs adipose tissue-derived human MSCs, MSCs mesenchymal stem cells, HLA-DR human leukocyte antigen-DR