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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Optimization of adipose tissue-derived mesenchymal stem cells by rapamycin in a murine model of acute graft-versus-host disease

Fig. 6

Analysis of T helper cell subsets in aGVHD mice administrated Ad-hMSCs or rapamycin-treated Ad-hMSCs. a Recipient BALB/c mice received 5 × 106 bone marrow cells and 1 × 107 splenocytes from B6 mice after lethal irradiation with 800 cGy. On day 1 post BMT, recipient mice received intravenous administration of Ad-hMSCs, rapamycin-treated Ad-hMSCs, or saline (control). Intracellular cytokine levels were determined in splenic CD4+ T cells by confocal microscopy on day 12 after BMT. Histopathology and confocal microscopic images were obtained for each mouse (n = 5), and representative images are shown. Bars represent the means ± SDs of data from five mice per group. ** P < 0.01; *** P < 0.001. The data are representative of at least three independent experiments. b The proportion of IFN-γ+ (mainly Th1), IL-4+ (mainly Th2), IL-17+ (mainly Th17), and CD25+Foxp3+ (mainly Treg) among splenic CD4+ T cells were assessed ex vivo by intracellular fluorescence-activated cell sorting. aGVHD acute graft-versus-host disease, Ad-hMSCs adipose tissue-derived human mesenchymal stem cells, BMT bone marrow transplantation

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