Fig. 2

Influence of medium, incubation time and MOI on transduction efficacy and eGFP production. a At both durations and MOI, maximum of eGFP-positive cells was found after transduction in HBSS and PBS. b eGFP production evaluated by MFI correlated with transduction efficacy and the amount of eGFP-positive cells showing PBS and HBSS to be similarly superior to DMEM and α-MEM. c Representative images of mADSC expressing eGFP 48 hours after being transduced by Bac-CE in α-MEM and HBSS. * p < 0.025 vs. DMEM and α-MEM under similar transduction conditions; & p < 0.05 vs. DMEM under similar transduction conditions; Mann–Whitney U test with Bonferroni correction. MOI multiplicity of infection, GFP green fluorescent protein, HBSS Hanks balanced salt solution, PBS phosphate-buffered saline, MFI mean fluorescence intensity, DMEM Dulbecco’s modified Eagle’s medium, α-MEM α-minimal essential medium, mADSC mouse adipose-derived stromal cells